Agarose matrix, SP group, fast flow, Equivalent to SP Sepharose FF
|Resin code||SP Seplife® FF|
|Appearance||White Sphere beads|
|Heat resistance||121℃, in water for 30mins|
|pH stability||4~13 (long term), 3~14 (Short term, CIP)|
|Application||Proteins, nucleic acids and peptides downstream|
of biopharmaceuticals and bioengineering
|Chemical stability||Stable in the following liquids: all common aqueous buffers; |
1 mol/L sodium hydroxide; 8 mol/L urea; 8 mol/L guanidine hydrochloride; 70% ethanol
|Adsorption (mg / ml)||>55mg/ml lysozyme/ml|
|Ion exchange capacity||0.18～0.25mmol /ml|
|Test conditions: Chromatography column 16mm * 300mm; Column bed height 15 cm; temperature 25℃; |
mobile phase was 0.1 mol / L NaCl.
Packing is performed in accordance with standard operating procedures. It must be ensured that each material is at operating temperature and that the gel needs to be degassed before it is packed.
Equilibrate the column with 2 to 5 column volumes of the loading balance, making sure that the conductivity and pH of the effluent are exactly the same as the conductivity and pH of the loading buffer.
The balance solution is a low concentration buffer solution such as NaOAC, PBS, and the like. A commonly used balance solution is 0.1 mol/L acetate buffer, pH 5.0.
(1)The sample is prepared with a balance solution, and the turbid sample is centrifuged and filtered for loading. Samples with too much salt concentration are processed and then dispensed.
(2)In general, the target product is combined on a column, the impurities are washed away with an equilibrium solution, and an eluent is selected to wash the target product.
(3)The extent to which the medium adsorbs the sample components depends on the charged nature of the sample, the ionic strength of the mobile phase, and the pH. The salt concentration is small, and the medium adsorbs to the sample components more firmly. When using SP chromatography media, the recommended pH is 1 unit above the isoelectric point of the target product.
Elution can be carried out by increasing the salt concentration or increasing the pH, and generally increasing the salt concentration. A commonly used eluent (solution B) such as: 0.1 mol/L acetate buffer + 2 mol/L NaCl, pH 5.0.
Generally, the buffer is washed with a high salt concentration (containing 1~2mol/L NaCl) or the pH is reduced by 10 times or more, and then washed with an equilibrium solution of the bound protein to balance.
If inactivated proteins or lipids are not washed off during regeneration, they can be removed by in-place cleaning (CIP).
(1)For proteins bound by ionic bonding, it can be removed with 0.5 to 1 column bed volume of 2 M NaCl.
(2) For precipitated proteins, hydrophobically bound proteins or lipids, it can be washed first with 1 column bed volume of 0.1 M NaOH, and then with an equilibrium buffer solution until the pH is neutral.
(3) For strongly hydrophobically bound proteins, lipids, etc., wash with 4 to 10 times the bed volume of 70% ethanol or 30% isopropanol. Note that the concentration of the organic solvent gradually increases in a gradient manner, otherwise it is easy Create bubbles.
Sealed and stored at 4~30℃(preservation solution is 20% ethanol + 0.2mol/L sodium acetate), dry, ventilated, clean place, can not be frozen;
Used column is stored at 4~8℃, 20% ethanol solution +0.2mol /L sodium acetate.
Sunresin has set up special topics and established a special research team based on customers’ needs to conduct research and analysis on the element composition and existing technology of tungsten leaching solution, and successfully developed sepcial resin Seplite® LSC680 for tungsten and molybdenum separation as well as the matching process.
The ion exchange resin (IER) method is very excellent and efficient for acetone purification. Acetone will pass cation exchange resins first and then the anion exchange resins, more than 95% methylamine will be removed.
On November 23, 2020，Forbes announced Asia’s Best Under A Billion 2019 list. Sunresin was honored to be selected. The promulgation ceremony originally scheduled to be held in Bangkok, Thailand, was carried out online due to continuous spreading pandemic.